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Reh 人急性非B非T**細(xì)胞白血病

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產(chǎn)品名稱: Reh 人急性非B非T**細(xì)胞白血病
產(chǎn)品型號: CRL-8286
產(chǎn)品廠商: 美國標(biāo)準(zhǔn)生物品收藏中心(ATCC)
產(chǎn)品文檔: 無相關(guān)文檔


簡單介紹

CRL-8286 Reh 人急性非B非T**細(xì)胞白血病,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!


Reh 人急性非B非T**細(xì)胞白血病 的詳細(xì)介紹
CRL-8286 Reh 人急性非B非T**細(xì)胞白血病
ATCC® Number: CRL-8286?    Price: $338.00
Designations: Reh
Depositors:  Univ. Pennsylvania
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast

Source: Disease: acute lymphocytic leukemia (non-T; non-B)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Antigen Expression: CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%)
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: 95% (v/v) FBS; 5% (v/v) DMSO
Storage temperature: Liquid nitrogen vapor phase
References: 22700: Greaves M, Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim. Biophys. Acta 516: 193-230, 1978. PubMed: 365240
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