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CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞

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產(chǎn)品名稱: CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞
產(chǎn)品型號: CCL-35
產(chǎn)品廠商: 美國標準生物品收藏中心（ATCC）
產(chǎn)品文檔: 無相關文檔


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CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！

CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞 的詳細介紹
CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞
ATCC® Number: CCL-35?    Price: $438.00
Designations: Pt K1 (NBL-3) [PTK1]
Depositors:  KH Walen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Potorous tridactylis (potoroo)
Morphology: epithelial
Source: Organ: kidney
Disease: normal
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Virus Resistance: poliovirus 2
Age:
Gender: female
Comments: The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; newborn bovine calf serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Rinse cell sheet twice with 0.25% trypsin, 0.03% EDTA (or Alsever's Trypsin Versene) solution, remove the trypsin solution, and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
References: 26188: . . Nature 194: 406, 1962.
26189: . . Cytogenetics 3: 19, 1964.
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