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CRL1472 HT-1376人膀胱癌細胞

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產(chǎn)品名稱: CRL1472 HT-1376人膀胱癌細胞
產(chǎn)品型號: CRL-1472
產(chǎn)品廠商: 美國標準生物品收藏中心(ATCC)
產(chǎn)品文檔: 無相關文檔


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CRL-1472 HT-1376 人膀胱癌細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*優(yōu)培養(yǎng)條件!


CRL1472 HT-1376人膀胱癌細胞 的詳細介紹
CRL-1472 HT-1376 人膀胱癌細胞
ATCC® Number: CRL-1472?    Price: $323.00
Designations: HT-1376
Depositors:  S Rasheed
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial

Source: Organ: urinary bladder
Tumor Stage: grade 3
Disease: carcinoma
Cellular Products: fibrinolytic activity; interferon
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 9,11
D16S539: 11,14
D5S818: 11,12
D7S820: 9,12
THO1: 7,10
TPOX: 8
vWA: 15,18
Cytogenetic Analysis: modal chromosome number = 104 to 121
Isoenzymes: G6PD, B
Age: 58 years
Gender: female
Ethnicity: Caucasian
Comments: The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens.
The cells will grow in soft agar.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks.
Related Products: purified DNA:ATCC CRL-1472D
References: 22538: Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628
23134: Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805
25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
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